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Prediction of centrifugation times for equilibrium and velocity sedimentation on various gradients
Authors:D B Bylund  G Bruening
Institution:1. Department of Biological Chemistry, University of California, Davis, California 95616 USA;2. Department of Biochemistry and Biophysics, University of California, Davis, California 95616 USA
Abstract:Computer and calculator programs have been prepared which can predict sedimentation times for a significant fraction of the kinds of density gradient centrifugation experiments currently being carried out in biochemistry. Gradients of sucrose, glycerol, or CsCl were accommodated for linear or several forms of convex or concave gradient profiles. Times of sedimentation to various uniformly spaced points between the meniscus and the bottom of the tube, or to a point near the isopycnic density, are predicted.
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