Evaluation Antimicrobial and Antiadhesive Properties of the Biosurfactant Lunasan Produced by <Emphasis Type="Italic">Candida sphaerica</Emphasis> UCP 0995 |
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Authors: | Juliana M Luna Raquel D Rufino Leonie A Sarubbo Lígia R M Rodrigues José A C Teixeira Galba M de Campos-Takaki |
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Institution: | 1.Universidade Federal de Pernambuco, Ciências Biológicas,Recife,Brazil;2.Universidade Federal de Pernambuco, Biologia de Fungos,Recife,Brazil;3.Universidade Católica de Pernambuco, Núcleo de Pesquisas em Ciências Ambientais,Recife,Brazil;4.Instituto de Biotecnologia e Bioengenharia, Universidade do Minho,Largo do Pa?o,Portugal |
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Abstract: | Different groups of biosurfactants exhibit diverse properties and display a variety of physiological functions in producer
microorganisms; these include enhancing the solubility of hydrophobic/water-insoluble compound, heave metal binding, bacterial
pathogenesis, cell adhesion and aggregation, quorum sensing and biofilm formation. Candida sphaerica was grown in a low cost medium, consisting of distilled water supplemented with 9% refinery residue of soybean oil and 9%
corn steep liquor, for 144 h at 28°C and 150 rpm. The cell-free supernatant obtained at the end of the experiments was submitted
to extraction, and afterward the biosurfactant was isolated using methanol with a yield of 9 g l−1. The critical micelle concentration of the biosurfactant was found to be 0.25 mg ml−1 with a surface tension of 25 mN m−1. Several concentrations of the biosurfactant (0.625–10 mg ml−1) were used to evaluate its antimicrobial and antiadhesive activities against a variety of microorganisms. The biosurfactant
showed antimicrobial activity against Streptococcus oralis (68%), Candida albicans (57%), and Staphylococcus epidermidis(57.6%) for the highest concentration tested. Furthermore, the biosurfactant at a concentration of 10 mg ml−1 inhibited the adhesion between 80 and 92% of Pseudomonas aeruginosa, Streptococcus agalactiae, Streptococcus sanguis12. Inhibition of adhesion with percentages near 100% occurred for the higher concentrations of biosurfactant used. Results gathered
in this study point to a potential use of the biosurfactant in biomedical applications. |
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