Glucocorticoids and the isolated rat hepatocyte |
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| Authors: | C J Lovell-Smith P Garcia-Webb |
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| Institution: | 1. Sustainable Biofuels and Coproducts Research Unit, Eastern Regional Research Center, United States Department of Agriculture, Agricultural Research Service, 600 East Mermaid Lane, Wyndmoor, PA 18951, USA;2. Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 9, 8092 Zurich, Switzerland;3. Food Quality Laboratory, Beltsville Agriculture Research Center, United States Department of Agriculture, Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, MD 20705, USA;4. Functional Foods Research, National Center for Agricultural Utilization Research, United States Department of Agriculture, Agricultural Research Service, 1815 N. University Street, Peoria, IL 61604, USA;5. Food Components and Health Laboratory, Beltsville Human Nutrition Research Center, United States Department of Agriculture, Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, MD 20705, USA;6. Center For Food Safety and Applied Nutrition, U.S. Food and Drug Administration, 5001 Campus Dr, College Park, MD 20740, USA |
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| Abstract: | Following incubation at 37 degrees C with tritiated glucocorticoids isolated hepatocytes prepared from non-adrenalectomized rats show rapid uptake of label. Uptake is non-saturable, and non-linear over the first 60 sec of exposure to steroids. HPLC separation of aqueous extracts of cells and incubation medium shows that polar metabolites of the natural steroid, corticosterone, appear within 10 sec, whereas the synthetic glucocorticoid, dexamethasone, is not altered. Our results suggest that diffusion is the most important process by which glucocorticoids enter liver cells, and that the predominant fate of corticosterone is rapid metabolism. |
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