Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification |
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Authors: | Luhe Annette Lin Tan Lily Wu Jinchuan Zhao Hua |
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Affiliation: | (1) Industrial Biotechnology Program, Institute of Chemical and Engineering Sciences, Agency for Science, Technology and Research (A*STAR), 1 Pesek Road, Jurong Island, 627833, Singapore; |
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Abstract: | Saccharomyces cerevisiae was transformed for higher ethanol tolerance by error-prone whole genome amplification. The resulting PCR products were transformed back to the parental strain for homologous recombination to create a library of mutants with the perturbed genomic networks. A few rounds of transformation led to the isolation of mutants that grew in 9% (v/v) ethanol and 100 g glucose l−1 compared to untransformed yeast which grew only at 6% (v/v) ethanol and 100 g glucose l−1. |
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