铁胁迫对微囊藻毒素合成酶McyC和McyI表达的影响

为研究微囊藻毒素合成酶基因的蛋白表达水平与环境因子间的关系,文章以位于微囊藻毒素合成基因簇两个操纵子中的mcyC和mcyI基因为代表,利用制备的高效McyC和McyI多克隆抗体,采用Western Blot技术检测了铁胁迫对微囊藻毒素合成酶McyC和McyI蛋白表达水平的影响。研究结果表明,在铁胁迫下,铜绿微囊藻PCC 7806藻细胞内McyC和McyI的蛋白水平变化趋势一致,且与相同条件下藻细胞内毒素的合成产量变化一致,暗示铁胁迫直接通过影响微囊藻毒素合成酶的表达水平调控毒素的合成。研究为进一步了解微囊藻毒素的合成机制提供了基础材料。

IRON STRESS ON THE EXPRESSION OF MICROCYSTIN SYNTHETASE MCYC AND MCYI

Microcystin is a class of cyclic hepatotoxin, which poses a great threat to human and animal health. Microcystin synthesis is regulated by a variety of environmental factors, and the synthesis efficiency of microcystin is directly determined by the amount of the corresponding synthetase and the catalytic rate, however, the relationship between protein expression levels of the microcystin synthesis gene cluster and environmental factors is still unclear. In this study, the mcyC and mcyI genes located in the two operons of the microcystin synthesis gene cluster were selected as representatives, using high-efficiency McyC and McyI polyclonal antibodies, detected the effect of iron stress on microcystin synthetase McyC and McyI protein expression levels by Western Blot. The result indicated that the protein levels of McyC and McyI within Microcystis aeruginosa PCC 7806 were consistent with the changes in the synthesis yield of toxins in vivo under iron stress, suggested that iron stress directly regulates the synthesis of the toxin by influencing the expression level of microcystin synthetase. This study provided the basis for further understanding the synthesis mechanism of microcystin.