Development of multiple elisas for the detection of antibodies against classical swine fever virus in pig sera |
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Authors: | Zhen-hua Yang Ling Li Zi-shu Pan |
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Institution: | State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China |
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Abstract: | The major immunogenic proteins (Erns, E2 and NS3) of classical swine fever virus (CSFV) (Shimen strain) were expressed in E. coli and purified by affinity chromatography. The recombinant antigens were applied to develop multiple enzyme-linked immunosorbent
assays (ELISAs) for the detection of specific antibodies in pig sera. Optimum cut-off values were determined by receiver operating
characteristic (ROC) analysis after testing 201 sera of vaccinated pigs and 64 negative sera of unvaccinated piglets. The
multiple ELISAs were validated with 265 pig sera yielding high sensitivity and specificity in comparison with the virus neutralization
results. The results demonstrated that multiple ELISAs can be a valuable tool for the detection of CSFV infection and serological
surveys in CSFV-free countries or for the evaluation of the antibody responses in pigs induced by a live attenuated C-strain
vaccination. |
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Keywords: | Classical swine fever virus Recombinant antigens ELISA |
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