黑木耳多糖对内毒素诱导急性肾脏损伤的保护作用及机制研究

目的:探讨黑木耳多糖对内毒素(LPS)诱导大鼠急性肾损害的保护效应及可能机制。方法:健康雄性SD大鼠,分为正常对照组、LPS组和黑木耳多糖组(LPS+黑木耳多糖)。根据分组,分别于复制模型前7 d给予生理盐水或1%黑木耳多糖(15 m L/kg)预防性灌胃。第8 d腹腔注射生理盐水或LPS(8 mg/kg),造模12 h后,通过腹主动脉采血检测血清尿素氮(BUN)、肌酐(Cr)水平,取肾脏制备匀浆检测肾组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)水平。结果:用黑木耳多糖干预后,大鼠血清中BUN、Cr水平均显著低于LPS组(P0.05);LPS组MDA含量较正常对照组增高,SOD、T-AOC水平降低,差异有统计学意义(P0.05);黑木耳多糖组MDA含量较LPS组降低,SOD、T-AOC水平升高,差异有统计学意义(P0.05)。结论:黑木耳多糖能显著提高内毒素血症大鼠的抗氧化能力,对肾脏组织有保护作用。

Auricularia Auricular-judae Polysaccharide AttenuatesLipopolysaccharide-induced Acute Kidney Injury by Inhibiting OxidativeStress

Objective:To investigate the role of Auricularia auricular-judae polysaccharide (AAP) in acute kidney injury inducedby lipopolysaccharide (LPS) in rats and to further explore the possible underlying mechanisms.Methods:Adult Sprague-Dawley ratswere randomly assigned into the control, LPS, and LPS plus AAP groups. Rats were injected with LPS (10 mg/kg, i.p.) to induce acutekidney injury. Rats in the LPS plus AAP group were treated with AAP for 7 days before the induction of ALI. Blood urea nitrogen(BUN)and creatinine (Cr) in serum were measured. Superoxide dismutase(SOD), total antioxidant capacity (T-AOC)activity and malondialdehyde(MDA) level in kidney were measured. Pathological changes of kidney tissues were observed by HE staining.Results:The datashowed that treatment with AAP group significantly improved LPS-induced lung pathological changes, decreased the BUN and Cr level,and reduced MDA level, increased the SOD, T-AOC activity.Conclusion:The results indicated that AAP has a protective effect onLPS-induced acute kidney injury in rat.