CD11c在新生儿及成人指皮组织表达的免疫组织化学研究

目的:研究新生儿及成人指皮组织CD11c阳性细胞的数量、形态和分布情况。方法:取7例尸检新生儿及5例成人无名指指皮组织样本,CD11c标记,行免疫组织化学染色,光镜观察阳性细胞的表达、分布情况并进行统计学分析。结果:人指皮组织中,CD11c阳性细胞和阳性突起呈棕褐色,胞体大小不等、形态多样;胞核呈圆形或椭圆形,突起长短粗细不等,可相互连接。阳性细胞呈散在或灶带状分布,灶带状分布常见于表皮乳头周围、血管神经分叉周围,血管外膜、环层小体被囊结缔组织内可见散在的CD11c阳性细胞。新生儿组CD11c阳性细胞的表达率为(31.0±9.4)%,高于成人组(6.0±2.7)%(P0.01)。结论:CD11c分子可能也是人指皮组织细胞分化过程中的阶段性标志物,其表达量的降低与年龄的增长有关。

Immunohistochemistry Study of Expression of CD11c In Finger Tissues of Neonates and Adults

Objective:To study the form, number and distribution of CD11c positive cells in the finger tissues of neonates andadults.Methods:Autopsy samples of ring finger tissues of seven neonates and five adults were stained with the method of immunohistochemistrystained with CD11c, which were then observed by light microscope and with statistical analysis.Results:CD11c+ cells andprocesses were colored with brown, which were different in sizes and shapes with their nucleus round or ellipse and with their processesdifferent in length and thickness, which also can be connected with each other. CD11c+ cells at scattering or focal or band- like distributioncould be seen in finger tissues, including the surrounding of epidermal pinning processes and in the branching of vessels and nerves.CD11c+ cells at scattering distribution could also be seen in adventitia and capsule tissues of Pacinian corpuscles. The expression ratio ofCD11c+ cells in neonates group is (31.0± 9.4) %, which is, higher than that of adults group (6.0± 2.7) %(P<0.01).Conclusion:CD11cmolecule is likely to be the periodic markers in the cell differentiation process of the finger skin tissue in human, the decreasing of CD11cexpression in human finger skin is related to the age growth.