绞股蓝多糖对糖尿病肾病大鼠肾功能的保护作用及其机制研究 |
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引用本文: | 杜小燕 覃华 田雯 张志武 侯颖. 绞股蓝多糖对糖尿病肾病大鼠肾功能的保护作用及其机制研究[J]. 现代生物医学进展, 2016, 16(22): 4247-4250 |
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作者姓名: | 杜小燕 覃华 田雯 张志武 侯颖 |
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作者单位: | 第四军医大学唐都医院药剂科;第四军医大学药学系药理学教研室 |
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基金项目: | 陕西省社会发展科技攻关项目(2015SF196) |
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摘 要: | 目的:探讨绞股蓝多糖对糖尿病大鼠肾功能的影响及其保护的可能机制。方法:采用大鼠高脂高糖饲料结合腹腔注射链脲佐菌素建立2型糖尿病模型。灌胃给予绞股蓝多糖8周后,测定大鼠空腹血糖(FBG),血清肌酐、血清尿素氮、24 h尿量及尿蛋白等指标;组织病理切片观察肾形态,肾小球体积;Western blot法检测肾皮质NF-κB的表达。结果:绞股蓝多糖(GPS)可剂量依赖性的降低DN组的FBG、血肌酐、24 h尿量和尿蛋白;但血尿素氮模型组与GPS组无统计学差异;中、高剂量GPS治疗后,肾脏指数较模型组明显降低(P0.05);与模型组(149.8±12.2%)比较,高剂量GPS可抑制肾小球体积肥大至108.9±9.6%。进一步研究发现,GPS可剂量依赖性的降低DN组肾脏NF-κB蛋白的表达,高、中剂量组水平与模型组相比有统计学差异(P0.05)。结论:绞股蓝多糖对实验性糖尿病肾病具有保护作用,其机制可能与抑制肾炎症相关通路NF-κB的表达有关。
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关 键 词: | 绞股蓝;多糖;糖尿病肾病;NF-kB |
Protection of Renal Function by the Polysaccharide fromGynostemmapentaphyllumon Diabetic Nephropathy and Its Mechanism |
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Abstract: | Objective:To investigate the protective effects of polysaccharide from Gynostemma pentaphyllum on type 2 diabeticnephropathy and discuss the mechanisms.Methods:Diabetes rat model was induced by injection of streptozotocin after feeding high-fatand high-sugar diet. The rats were divided into six groups. After the rats were treated with Gynostemma pentaphyllum polysaccharide orpositive control agent, enalapril for 8 weeks, the changes of fasting blood glucose (FBG), 24 h urine, urine protein and other biologicalindexes were assessed. The pathological changes in the kidney were determined by H&E staining. The expression of NF-kB was detectedby western blotting.Results:Compared with model group, administration of the polysaccharide from Gynostemma pentaphyllum (GPS)can reduce FBG, plasma creatinine, 24 h urinary volume and urine protein in a dose-dependent manner. But there was no significantdifference in blood urea nitrogen between model and GPS groups. After the 8-week treatment with medium or high dose of GPS, thekidney index was significantly decreased. High dose of GPS can decrease the glomerulus volume to 108.9 ± 9.6%, compared to thevalue of 149.8 ± 12.2% in model group. Furthermore,the expression of NF-kB protein in renal tissues was also significantly decreased.Conclusion:These data indicate that Gynostemma pentaphyllum polysaccharide protects renal function in rats of type 2 diabetes. Themechanismmay be related with the inhibition of inflammation via NF-kB signaling pathway. |
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Keywords: | Gynostemma pentaphyllum Polysaccharide Diabetic nephropathy NF-kB |
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