长链非编码RNA Dleu2 对宫颈癌细胞增殖、迁移及侵袭的影响

目的:研究长链非编码RNA(long non-coding RNA,lnc RNA)Dleu2对宫颈癌细胞增殖、迁移和侵袭能力的影响。方法:利用高通量Lnc RNA芯片技术检测10例宫颈癌组织及对应的癌旁组织,筛选得到一批表达水平具有显著差异的Lnc RNA,进一步针对可能具有生物学功能的Lnc RNA-Dleu2,利用q-PCR验证其在癌组织样本中的相对低表达。再通过在细胞内过表达Lnc RNA-Dleu2研究其对宫颈癌Hela和Caski细胞系增殖、迁移和侵袭的影响。结果:q-PCR结果验证了Lnc RNA芯片筛选的结果,即相较于癌旁组织和正常宫颈上皮细胞系,Lnc RNA-Dleu2在宫颈癌组织和细胞中均低表达。CCK8和克隆形成实验结果显示,过表达Lnc RNA-Dleu2能显著抑制Hela和Caski细胞增殖能力(P0.01);细胞划痕实验结果显示,过表达Lnc RNA-Dleu2能显著抑制Hela和Caski细胞增殖和迁移能力;Matrigel细胞侵袭实验结果显示,过表达Lnc RNA-Dleu2能显著抑制Hela和Caski细胞的侵袭能力(P0.01)。结论:Lnc RNA-Dleu2在宫颈癌中相对低表达,提高Dleu2表达水平能够抑制宫颈癌细胞系Hela和Caski的增殖、迁移和侵袭能力。

Effect of lncRNA Dleu2 on Proliferation, Migration and Invasion of CervicalCancer Cells

Objective:To investigate the potential biological effects of long non-coding RNA Dleu2 on the characteristics of cervicalcancer cells, including items of proliferation, migration and invasion.Methods:High throughput microarray screening of LncRNAwas performed with 10 different cervical cancer tissues as samples and corresponding para-carcinoma tissues as controls, LncRNAs withsignificantly different expression levels were selected and among which, the microarray results of LncRNA Dleu2 was further verified byq-PCR method, then the overexpression of Dleu2 in Hela and Caski, were performed to investigate its potential functions on the proliferation,migration and invasion of these two cervical cancer cell lines, which characteristic items were quantified separately by CCK8,clone-forming unit assay, wound healing assay and tumor invasion assay methods.Results:Both the results of Microarray and q-PCRdemonstrated that the expression level of Dleu2 was significantly much lower in cervical cancer tissues and cell lines as compared withcorresponding normal controls. The results of CCK8 assay and colony-forming unit assay showed that Dleu2 overexpression could significantlydecrease cervical cancer cell proliferation. Wound Healing assay results indicated that Dleu2 overexpression could obviously suppresscervical cancer cell proliferation and migration. Tumour invasion assay results demonstrated that Dleu2 could significantly suppresscervical cancer cell invasion.Conclusion:The expression levels of Dleu2 are relatively much low in cervical cancer tissues and cell lines.The overexpression of Dleu2 can significantly inhibit the ability of proliferation,migration and invasion of Hela and Caski cells.