血管紧张素-II（Ang-II）处理后的人脐带MSCs上清液对HUVEC 凋亡增殖的影响

目的:体外观察100 ng/m L血管紧张素-Ⅱ(Angiotensin-Ⅱ,Ang-Ⅱ)处理后的人脐带间充质干细胞(human umbilical cord MSCs,h UCMSCs)上清液对损伤的人脐静脉内皮细胞(human umbilical vein endothelia cells,HUVEC)增殖、凋亡的影响。方法:CCK8法检测HUVEC增殖情况;倒置显微镜下观察HUVEC形态;吖啶橙/溴化乙锭染色法(acridineorange/ethidium bromide,AO-EB)、Hoechst检测HUVEC凋亡情况。结果:CCK8实验结果显示,加入100 ng/m L Ang-Ⅱ处理后的h UCMSCs上清液组HUVEC增殖速度在各时间点显著快于其他两组。倒置显微镜下观察细胞的形态:加入100 ng/m L Ang-Ⅱ处理后的h UCMSCs上清液组HUVEC的形态最佳且凋亡数最少。用各组细胞上清液对HUVEC培养24 h、48 h、72 h后,AO-EB、Hoechst染色结果显示:加入100 ng/m L Ang-Ⅱ处理后的h UCMSCs上清液组,在各个时间点HUVEC出现凋亡细胞或死亡细胞的数最少。结论:100ng/m L Ang-Ⅱ预处理后的h UCMSCs上清液可以促进HUVEC增殖、抑制HUVEC凋亡。

Effects of Conditioned MediumDerived fromMesenchymal StemCellsPretreated with Angiotensin-II on Apoptosis and Proliferation of HumanUmbilical Vein Endothelia Cells

Objective:To investigate the effects of conditioned medium derived from mesenchymal sterm cells (hUCMSCs)pretreated with 100 ng/mL Ang-II on the proliferation and apoptosis of human umbilical vein endothelia cells (HUVEC) in vitro.Methods:The cell proliferation curve was measured by CCK8, The cell morphology and density were observed by inverted microscope,and the apoptosis was assessed by AO-EB staining and Hoechst staining.Results:The results of CCK8 showed that the Cells value-addupof 100 ng/mL Ang-II pretreatment was significantly faster than the other two groups at every time point; the morphology of the cells wasobserved under an inverted microscope, The results showed the morphology of HUVEC in the supernatant of 100 ng/mL was the best andthe number of apoptosis was the least. The HUVEC were cultured in the conditioned medium of each group after 24 h, 48 h and 72 h,The results of AO-EB and Hoechst staining shows the number of apoptotic cells or dead cells in group of 100 ng/mL Ang-II pretreatmentwas the least at each time point.Conclusion:The conditioned medium of hUCMSCs which pretreated with 100 ng/mL Ang-II promotedHUVEC proliferation and inhibited HUVEC apoptosis.