CREG促进小鼠腹腔巨噬细胞溶酶体发生及溶酶体组织蛋白酶表达*

目的:研究E1A激活基因阻遏子(Cellular repressor of E1A-stimulated genes,CREG)对小鼠腹腔巨噬细胞溶酶体发生及溶酶体组织蛋白酶表达的影响。方法:应用loss-of-function和gain-of-function模型,Lysotracker Red染色和透射电镜观察CREG对小鼠腹腔巨噬细胞溶酶体发生的影响,并通过细胞免疫荧光染色和Western blot方法,观察CREG对小鼠腹腔巨噬细胞溶酶体组织蛋白酶表达的影响。结果:Lysotracker Red染色和透射电镜证实CREG可促进小鼠腹腔巨噬细胞溶酶体发生;细胞免疫荧光染色和Western blot方法证实CREG可促进小鼠腹腔巨噬细胞溶酶体组织蛋白酶cathepsin B及cathepsin S表达。结论:CREG可促进小鼠腹腔巨噬细胞溶酶体发生及组织蛋白酶cathepsin B及cathepsin S表达。

CREG Promotes Lysosome Biogenesis and Expression of Cathepsins inMouse Peritoneal Cavity Macrophages

Objective:To study the effects of cellular repressor of E1A stimulated genes (CREG) on lysosome biogenesis andexpression of cathepsins in mouse peritoneal cavity macrophages.Methods:Lysotracker Red staining and transmission electronmicroscope were applied to observe the effect of CREG on lysosome biogenesis in mouse peritoneal cavity macrophages usinggain-of-function and loss-of-function approaches. The effect of CREG on expression of cathepsins in mouse peritoneal cavitymacrophages were studied by immunofluorescence staining and Western blot.Results:It was confirmed that CREG can promotelysosome biogenesis in mouse peritoneal cavity macrophages using Lysotracker Red staining and transmission electron microscope. Thefindings of immunofluorescence staining and Western blot provided evidence that CREG promoted expression of cathepsin B andcathepsin S in mouse peritoneal cavity macrophages.Conclusion:CREG promotes lysosome biogenesis and expression of cathepsin Band cathepsin S in mouse peritoneal cavity macrophages.