钛- 载药纳米复合材料的组装及其性质的研究

目的:构建一种能结合钛表面的载药纳米粒及钛-载药纳米复合材料的组装和性质研究。方法:(1)多巴胺修饰的非离子表面活性剂多巴胺-泊洛沙姆188(Dop-Poloxamer188)的合成和检测;(2)Dop-Poloxamer188作为表面活性剂、PLGA作为油相基质,制备纳米粒及纳米粒载药和表征;(3)钛片的预处理及钛片与修饰后的纳米粒的结合;(4)纳米粒修饰后的钛表面的表征。结果:新合成的Dop-Poloxamer188在285 nm左右有紫外吸收峰,说明多巴胺成功的修饰在Poloxamer188的两端;Dop-Poloxamer188能和PLGA制备出很好的纳米粒,平均粒径在110 nm左右,PDI小于0.1;多巴胺修饰的纳米粒与钛片通过简单的浸渍过程结合后,通过水接触角、场发射扫面电镜(Fe-SEM)、荧光显微镜、X射线光电子能谱(XPS)等仪器检测都显示多巴胺修饰的纳米粒成功且牢固的修饰在钛片表面。结论:成功达到钛表面的载药纳米粒修饰的目的,为钛种植体的载药系统提供了新的思路和方法。

The Characterization of a Selfassembly Titanium/drug-loading Nanoparticles Complex

Objective:To prepare and utilize a novel Titanium surface affinity drug loaded nanoparticles to build a Titanium/ nanoparticle complex which could be used for drug delivery and release.Methods:(1) The synthesis and characterization of dopamine modified non-ionic surfactants, Dop-Poloxamer188. (2) Using Dop-Poloxamer188 as a surfactant, PLGA as oil phase matrix and model drugs, drug-loaded nanoparticles were prepared and characterized. (3) With pretreatment of the titanium plate, Dop-functionalized nanoparticles and titanium plate selfassembled into a Titanium/nanoparticles (Ti/NP). (4) The characterization of Ti/NP complex.Results:The new compound Dop - Poloxamer188 had an ultraviolet absorption peak at about 285 nm, indicating that the successful modification of dopamine to Poloxamer188; the Dop-Poloxamer188-PLGA nanoparticels had an average diameter of around 110 nm with polydispersity index (PDI) less than 0.1. Furthermore, Dopamine modified nanoparticles were immobilized on titanium surface through a simple dipping process. Water contact angle test, field-emission scanning electron microscope (Fe-SEM), fluorescence microscopic analysis and X-ray photoelectron spectroscopy (XPS) detection confirmed that dopamine modified nanoparticles were immobilized on titaniumsurface.Conclusion:This study successfully constructed a novel drug-loaded nanoparticles which could be immobilized on titanium surface. The novel drug loaded Ti/NP complex had the potential to be used for site specific drug delivery and controlled release in the broad area of Ti based biomaterials.