紫草素对人脑胶质瘤U251 细胞增殖和凋亡的影响

目的:观察紫草素对体外培养的人脑胶质瘤U251细胞的增殖和凋亡的影响。方法:应用MTT法和Annexin V-FITC/PI双染流式细胞术分别检测2.5、5、10μM/L的紫草素对U251细胞的体外抑杀作用以及凋亡诱导作用,进一步应用Western blot方法检测紫草素对凋亡相关蛋白Bcl-2及Bax表达水平的影响。结果:紫草素对人U251胶质瘤细胞具有明显的增殖抑制作用,且呈一定的剂量依赖性和时间依赖性。紫草素可明显上调U251细胞Bax的表达,下调Bcl-2的表达,与对照组相比存在显著性差异(P0.05)。结论:紫草素对人胶质瘤U251细胞具有明显的抑制增殖和促进凋亡作用。

Study on U251 Glioma Cell Proliferation and Apoptosis induced by Shikonin

Objective:To study the effect of shikonin on the proliferation and apoptosis of U251 glioma cells in vitro.Methods:MTT assay and AnnexinV-FITC/PI double staining flow cytometry were used to test the cell proliferation and apoptosis induced by 2.5, 5, 10 uM/L shikonin on U251 cells. Western blot was used to detect apoptosis-related protein Bcl-2 and Bax expression levels.Results:Shikonin exhibited prominent inhibition effect on U251 cells proliferation in a dose-dependent and time-dependent manner. Shikonin significantly upregulated the expression of Bax and downregulated the expression of Bcl-2.Conclusion:Shikonin could significantly inhibit the proliferation and promote apoptosis on human glioma U251 cells.