PCT及CRP在急性肠梗阻大鼠血清中的表达水平及其临床意义

目的:探讨降钙素原(Procalcitonin,PCT)及C反应蛋白(C-reactive protein,CRP)在急性肠梗阻大鼠血清中的水平及其临床意义。方法:将83只Wistar大鼠分为对照组(n=13)、假手术组(n=35)和急性肠梗阻组(n=35)。对照组大鼠采集标本后处死,肠梗阻组行开腹手术结扎回肠末端,假手术组仅行开腹手术。检测8 h、24 h、48 h、72 h及96 h血清PCT及CRP水平,观察急性肠梗阻大鼠回肠组织的病理学改变情况。结果:假手术组PCT与CRP水平在术后24 h内显著升高,48 h至96 h逐渐下降;各时间点PCT水平明显高于对照组,而CRP水平在实验结束时已恢复至正常水平。肠梗阻组PCT和CRP水平在各时间点均明显高于对照组,并逐渐增加,到实验结束时达到高峰;肠梗阻组PCT和CRP水平在48 h-96 h均显著高于假手术组,差异具有统计学意义(P0.05)。组织病理学检查显示,对照组大鼠肠壁粘膜结构正常,假手术组可见轻度病理改变,肠梗阻组大鼠回肠组织可见粘膜结构明显破坏,绒毛坏死,严重水肿和炎症细胞浸润。结论:血浆PCT和CRP水平能够反映肠梗阻的状态和肠粘膜受损程度。

Expression of PCT and CRP in the Plasma and SerumTissues of Rats withAcute Intestinal Obstruction and Its Clinical Significance

Objective:To investigate the level and clinical significance of procalcitonin (PCT) and C-reactive protein (CRP) inplasma of rats with acute intestinal obstruction.Methods:83 Wistar rats were randomly divided into control group (n=13), shamoperation group (n=35) and acute intestinal obstruction group (n=35). The rats in control group were sacrificed, the rats in the shamgroupwere only taken the open surgery, and the rats in the intestinal obstruction group were taken the open surgery and the ligation of theterminal ileum. The serum levels of CRP and PCT were detected at different time points, and the pathological changes of ileum in ratswith acute intestinal obstruction were observed.Results:The level of PCT and CRP in sham operation group was gradually increased at 8h and 24 h and decreased gradually at 48 h, 72 h and 96 h, and the level of PCT was higher than that of control group at different timepoints, but the level of CRP at 96 h was returned to control. The level of PCT and CRP in intestinal obstruction group at different timepoints were significantly higher than those of the control group, and it was gradually increased to the peak at the end of the experiment,and the level of PCT and CRP in intestinal obstruction group at 48 h, 72 h and 96 h were significantly higher than those in shamoperationgroup with statistical significance (P<0.05). Histopathological examination showed that the structure of the intestinal wall in the controlgroup was normal, and mild pathological changes were detected in the sham operation group, and the ileum tissues of the intestinalobstruction group were obviously damaged, with the villi necrosis, severe edema and inflammatory cell infiltration.Conclusion:Thelevels of PCT and CRP can reflect the state of intestinal obstruction and the extent of intestinal mucosal damage.