夹竹桃麻素对双酚a诱导的成年雄性小鼠精子损伤作用的实验研究

目的:研究NADPH氧化酶抑制剂夹竹桃麻素(Apocynin)对双酚a(Bisphenol a,BPA)诱导的成年雄性小鼠精子损伤过程中的作用。方法:成年雄性小鼠给以BPA刺激,给以Apocynin(1 mg/kg/day和10 mg/kg/day,分别处理7 day)进行治疗,观察其对BPA诱导的成年雄性小鼠精子损伤的作用。结果:BPA处理组小鼠附睾精子数目和活力明显降低,血清中睾酮和黄体生成素水平明显减少;Apocynin治疗明显增加BPA处理组小鼠附睾中精子数目和活力,但是对血清中睾酮和黄体生成素水平没有明显影响。另外,BPA处理组小鼠睾丸中丙二醛(Malonaldehyde,MDA)水平明显增加;Apocynin治疗明显抑制了BPA处理组小鼠睾丸中MDA水平。结论:NADPH氧化酶抑制剂Apocynin减轻BPA诱导的成年雄性小鼠精子损伤。

Apocynin can Alleviate Bisphenol A-induced Sperm Damage in Adult MaleMice

Objective:To investigate the effect of treatment with apocynin (one NADPH oxidase inhibitor) against biophenol a(BPA)-induced injury in sperms of male adult mice.Methods:Male adult mice were stimulated with BPA and simultaneously treatedwith apocynin (1 mg/kg, 10 mg/kg) for 7 days. Testis and serumwere collected for biochemical analysis.Results:Exposure to BPA led toreduced number and motility of sperms of epididymises and serum levels of testosterone and luteinizing hormone. Treatment of BPAmice with apocynin enhanced the number and motility of sperms of epididymises, but did not affect the serum levels of testosterone andluteinizing hormone. In addition, exposure to BPA led to enhanced formation of malondialdehyde in testis, and treatment of BPA micewith apocynin reduced formation of malondialdehyde in testis.Conclusion:Apocynin attenuated BPA-induced injury in sperms of maleadult mice.