miR-203在肝细胞肝癌中的表达及其对肿瘤细胞增殖及侵袭能力的影响

目的:探讨miR-203在肝细胞肝癌(Hepatocellular carcinoma,HCC)患者血浆中的表达及其对肝癌细胞增殖和侵袭能力的影响。方法:选择2013年5月-2015年7月在我院确诊为肝细胞肝癌的患者57例作为研究对象,另选取同期在我院接受健康体检的志愿者49例作为对照组。采用实时荧光定量RT-PCR法检测两组血浆中miR-203的表达水平;将过表达的miR-203转染至人肝癌细胞系Hep 3B和JHH-1;采用CCK-8法检测miR-203对肝癌细胞增殖能力的影响;采用transwell法检测miR-203对肝癌细胞迁移及侵袭能力的影响。结果:miR-203在肝癌患者血浆中的表达水平显著低于对照组,差异具有统计学意义(P0.01);与未转染的肝癌细胞比较,Hep 3B和JHH-1细胞转染miR-203mimic后,肿瘤细胞的增殖、迁移及侵袭能力受到明显抑制,差异具有统计学意义(P0.05)。结论:miR-203在肝细胞肝癌中呈低表达,而过表达miR-203能够抑制肿瘤细胞的增殖及侵袭能力,提示miR-203可以作为一种新的肿瘤抑制性micro RNA。

Expression and Effects of miR-203 on the Proliferation and Invasion of Hepatocellular Carcinoma Cells

Objective:To investigate the expression of miR-203 in HCC, and the effects on the proliferation and migration of tumor cells.Methods:57 cases with HCC who were diagnosed in our hospital from May 2013 to July 2015 were selected as the research objects, and another 40 healthy volunteers were chosen to be the healthy control group. The RT-PCR was used to detect the expressions of miR-203 in the two groups. Then the over-expressed miR-203 was transfected into the human hepatocellular carcinoma cell line hep 3b and JHH-1. The effect of miR-203 on the proliferation of hepatoma cells was detected by CCK-8 assay, and the effect of miR-203 on the migration and invasion was detected by Transwell assay.Results:The expression of miR-203 in HCC was significantly lower than that of the control group, and the difference was statistically significant (P<0.01). Overexpression of miR-203 in HCC cells Hep 3B and JHH-1 could inhibite the cells'' proliferation, migration and invasion, and the difference was statistically significant (P<0.01).Conclusion:The expressionsof miR-203 in HCC was lower and the overexpression of miR-203 in HCC cells can inhibite the cells proliferation, migration and invasion, which might be an tumor suppressive microRNA.