中空硅纳米药物递送体系的线粒体靶向性探究

目的:探究中空硅纳米粒在细胞线粒体内的定位。方法:以阿霉素为模型药物及荧光影像分子,通过激光共聚焦显微镜,考察中空硅纳米粒与线粒体分子探针在线粒体内的共定位。通过差速离心法提取线粒体,进一步确认硅纳米粒在线粒体内的聚集。结果:与线粒体分子探针为共定位参照,中孔硅纳米粒在线粒体中分布的皮尔逊系数为0.758±0.033,线粒体提取结果显示,进入细胞的纳米粒有90.01±3.89%分布于线粒体。结论:中空硅纳米粒具有自发聚集于线粒体的能力,可以作为药物靶向递送载体,为线粒体类疾病的治疗提供新的平台。

Hollow Silica Nanoparticles for Mitochondria-targeted Drug Delivery

Objective:To investigate the location of hollow silica nanoparticles in cells.Methods:Taken doxorubicin hydrochlorideas the modal drug and fluorescence imaging molecular, the location of silica nanoparticles in cells were co-located with Mito-trackerDeep Red by laser scanning confocal microscope. The location of silica nanoparticles were further confirmed by isolation of mitochondrialand cytosolic extracts through differential centrifugation.Results:Compared with Mitotracker Deep Red, the Pearson''s Coefficient of thesilica nanoparticles was 0.758± 0.033, while the isolation of mitochondria showed 90.01± 3.89% of the model drug distributed in mitochondria.Conclusion:The hollow silica nanoparticles can spontaneously gathered in mitochondria, which provides a new platform tomitochondria-targeted drug delivery systemfor mitochondria dysfunctional diseases.