低血压灌注对无心跳大鼠肺支气管肺泡灌洗液中不同白细胞及HMGB1 的影响

目的:研究低血压灌注对无心跳大鼠肺支气管肺泡灌洗液中不同白细胞及HMGB1的影响。方法:将32只180-200g SD雄性大鼠随机分成对照组sham]、缺血30min组30I]、缺血60 min组60I]和缺血90 min组90I](n=8),4组大鼠分别接受麻醉后持续机械通气,测定并统计各组支气管肺泡灌洗液中总白细胞计数及不同白细胞计数,免疫印迹法检测支气管肺泡灌洗液中HMGB1蛋白的表达。结果:总白细胞、巨噬细胞、淋巴细胞计数及HMGB1蛋白表达均随着低血压灌注时间的延长而逐渐增加,尤其在60I]和90I]两组,灌洗液中巨噬细胞和淋巴细胞计数有显著增高,并且巨噬细胞和淋巴细胞与HMGB1蛋白表达呈正相关。结论:本研究证实HMGB1蛋白表达和巨噬细胞,淋巴细胞计数在低血压灌注缺血期内升高,提示HMGB1蛋白可能具有驱动炎症反应的作用。

Impact of Hypotension Perfusion on Different Leukocytes in Bronchoalveolar Lavage From Non-Heart-Beating Rat Lung and HMGB1

Objective:Hypotension perfusion study on different leukocytes in bronchoalveolar lavage from non-heart-beating rat lung and HMGB1.Methods:32 SD rats (180g-200 g) that were randomly assigned to four groups that are control groupsham], ischemia group for 30, 60, 90 minutes (Groups 30I], 60I], 90I], n=8, respectively), which were continuous ventilating for 90min sham] after anesthesia in order to determine and calculate the total and various number of Leukocytes in each Bronchoalveolar Lavage with Western blot method to detect the expression of HMGB1 protein in bronchoalveolar lavage.Results:This study confirmed that the total cells, macrophages, lymphocytes, Leukocytes and HMGB1 numbers increased progressively with the rising of hypotension perfusion. Especially, a significant rise in macrophages and lymphocytes was observed between60I] and 90I]. A positive correlation was found in the number of macrophages and the number of lymphocytes and HMGB1 Protein Level.Conclusion:This study demonstrates for the first time that HMGB1 Protein Level and macrophages and lymphocytes increase significantly during hypotension perfusion ischemia. Prompting HMGB1 protein may have driven the role of inflammation.