miR-126 在膀胱癌患者尿液中的表达及临床意义

目的:探讨miR-126在膀胱癌患者尿液中的表达与临床病理特征的关系,评估miR-126的肿瘤标志物诊断价值。方法:收集48例初发膀胱尿路上皮癌患者与32例健康对照者晨尿,提取尿液总RNA,通过实时荧光定量PCR技术检测各样本中的miR-126的表达水平,并经受试者工作曲线(ROC)分析其诊断价值。结果:膀胱癌患者尿液中的miR-126表达水平相对健康对照组明显上调(P0.01),其表达水平在不同病理级别之间存在显著差异(P均0.05),且低级别组表达水平略高于高级别组,与肿瘤大小、数目以及淋巴转移也有一定的相关性(P0.05),而与患者的年龄、性别、TNM分期等均无相关性(P0.05)。通过ROC曲线分析尿液中miR-126诊断膀胱肿瘤的曲线下面积(AUC)为0.861,当最佳切点定在7.475时,miR-126诊断膀胱肿瘤的敏感性和特异性分别为75.0%、81.2%。结论:膀胱癌患者尿液中miR-126的表达差异能够反映病情进展程度,其表达水平对膀胱肿瘤的早期诊断及病情评估具有一定的价值。

Differential Expression and Clinical Significance of Urine miR-126 in Bladder Cancer

Objective:To investigate the relationship between urine miR-126 expression and the clinicopathological parameters of bladder cancer, and evaluate the diagnostic value of miR-126 as tumor marker.Methods:Urine miR-126 expression of 48 patients with bladder cancer and 32 cases of healthy controls were assayed by RT-PCR, total urine RNAs was extracted, and its association with clinical pathological features of bladder cancer was analyzed, and its diagnostic value was evaluated by the ROC curve.Results:In contrast to controls, the level of urine miR-126 of bladder cancer patients was significantly raised (P<0.01), however, it significantly negatively correlated with histologic grade (P<0.05). In addition, the expression of urine miR-126 was significantly related to tumor size, tumor number and lymph node metastasis(P<0.05), but not related to age, sex or TMNstage(P>0.05). Using ROC curve analysis the urine miR-126 in the diagnosis of bladder tumor AUC was 0.861, when set optimal cutoff point 7.475, the sensitivity and specificity of miR-126 in the diagnosis of bladder tumor was 75.0 %, 81.2% respectively.Conclusion:The expression levels of miR-126 in patients'' urine of bladder cancer were significantly increased and closely related to the degree of disease progression, indicating that miR-126 may play a role in the progress of occurrence and development of bladder cancer, and has the vital significance for early diagnosis of this disease.