小鼠去细胞拟胚体对小鼠Lewis肺癌细胞体内生长的影响

目的:阐明小鼠去细胞拟胚体对小鼠Lewis肺癌细胞在体内生长的影响。方法:先制备来源于小鼠胚胎干细胞的拟胚体,然后用SDS去细胞处理。实验分成3组:小鼠Lewis肺癌细胞与去细胞拟胚体培养组,癌细胞与Matrigel培养组和单纯癌细胞组(每组n=12)。培养3天后注射入裸鼠体内,观察肿瘤生长情况。28天取出瘤体,Ki67和CD31免疫组化染色(n=12)检测细胞增殖和肿瘤微血管密度(MVD),Western blot检测组织Paxillin,E-cadherin和β-actin水平(n=6)。结果:去细胞拟胚体组肿瘤生长明显较单纯细胞组和Matrigel组慢。去细胞拟胚体组Ki67指数((17.1±2.6)%)明显小于单纯细胞组((34.5±4.7)%)和Matrigel组((48.4±8.6)%)(P0.05);去细胞拟胚体组的MVD(18.7±3.6个/mm2)明显小于单纯细胞组(32.1±6.4个/mm2)和Matrigel组(42.6±7.1个/mm2)(P0.05)。Western blot结果提示去细胞拟胚体组的Paxillin表达小于单纯细胞组和Matrigel组(P0.05),而E-cadherin表达大于单纯细胞组和Matrigel组(P0.05)。结论:小鼠去细胞拟胚体对小鼠Lewis肺癌细胞在体内有明显的促分化作用。

Effect of Acellular Mouse Embryoid Bodies on Growth of Mouse Lewis LungCancer Cells in Vivo

Objective:To investigate the effect of mouse acellular embryoid bodies (EBs) on growth of mouse Lewis lung cancercells in vivo.Methods:Mouse acellular EBs were prepared by decellularization of mouse EBs with sodium dodecyl sulfate (SDS). MouseLewis lung cancer cells were seeded in the acellular EBs, with cultured in Matrigel and cultured at 2-dimension as controls (n=12 foreach group). After cultured for 3 days, cells were injected to 6-week old BALB/c (nu/nu) mice for subcutaneous tumor experiment. Tumorswere retrieved at 28-day implantation. Immunohistochemical staining (n=12) and western blot (n=6) were used to evaluate cell proliferation,microvessel density and protein expression levels.Results:The tumor volumes of acellular EBs group were less than those ofMatrigel group and 2-dimension control group (both P<0.05). Immunohistochemical staining showed Ki67 labeling index and microvesseldensity labeled by CD31 less than Matrigel group and 2-dimension control group (all P<0.05). Western blot presented the least Paxillinand the most E-cadherin expression for acellular EBs group.Conclusion:The acellular EBs promote differentiation of mouse Lewislung cancer cells in vivo.