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Direct sequencing of the human microbiome readily reveals community differences |
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| Authors: | Justin Kuczynski Elizabeth K Costello Diana R Nemergut Jesse Zaneveld Christian L Lauber Dan Knights Omry Koren Noah Fierer Scott T Kelley Ruth E Ley Jeffrey I Gordon Rob Knight |
| Affiliation: | 1. Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO, 80309, USA 2. Department of Microbiology and Immunology, Stanford University, Stanford, CA, 94305, USA 3. Institute of Arctic and Alpine Research (INSTAAR), University of Colorado, Boulder, CO, 80309, USA 4. Cooperative Institute for Research in Environmental Sciences (CIRES), University of Colorado, Boulder, CO, 80309, USA 5. Department of Computer Science, University of Colorado, Boulder, CO, 80309, USA 6. Department of Microbiology, Cornell University, Ithaca, NY, 14853, USA 7. Department of Biology, San Diego State University, San Diego, CA, 92182, USA 8. Center for Genome Sciences, Washington University School of Medicine, St Louis, MO, 63108, USA 10. Howard Hughes Medical Institute, University of Colorado, Boulder, CO, 80309, USA 9. Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO, 80309, USA |
| Abstract: | Culture-independent studies of human microbiota by direct genomic sequencing reveal quite distinct differences among communities, indicating that improved sequencing capacity can be most wisely utilized to study more samples, rather than more sequences per sample. |
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