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High prevalence of autoantibodies to RNA helicase A in Mexican patients with systemic lupus erythematosus |
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| Authors: | Monica Vázquez-Del Mercado Claudia A Palafox-Sánchez Jose F Muñoz-Valle Gerardo Orozco-Barocio Edith Oregon-Romero Rosa E Navarro-Hernández Mario Salazar-Páramo Juan Armendariz-Borunda Jorge I Gámez-Nava Laura Gonzalez-Lopez Jason YF Chan Edward KL Chan Minoru Satoh |
| Affiliation: | 1. Departamento de Biología Molecular y Genómica, Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Guadalajara, Jalisco, CP 44340, México 2. División de Medicina Interna, Departamento de Reumatología, Hospital Civil 'Dr. Juan I. Menchaca', Salvador de Quevedo y Zubieta N° 750, CP 44340, Guadalajara, Jalisco, México 3. Departamento de Inmunología y Reumatología del Hospital General de Occidente, Secretaría de Salud Jalisco, Av. Zoquipan 1050, Jalisco, CP 45100, Zapopan, México 4. División de Investigación, Hospital de Especialidades, CMNO, IMSS, and Departamento de Fisiología, CUCS, Universidad de Guadalajara, Sierra Mojada 950, CP 44340, Guadalajara, Jalisco, México 5. Departamento de Biología Molecular y Genómica, Instituto de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, CP 44340, Guadalajara, Jalisco, México 6. Servicio de Medicina-Interna-Reumatología, Hospital Regional 110, IMSS, Guadalajara, Jalisco, México 7. Division of Rheumatology and Clinical Immunology, Department of Medicine, University of Florida, 1600 SW Archer Road, Gainesville, FL, 32610-0424, USA 8. Department of Oral Biology, University of Florida, 1600 SW Archer Road, Gainesville, FL, 32610-0424, USA 9. Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, 1600 SW Archer Road, Gainesville, FL, 32610-0221, USA |
| Abstract: | IntroductionAutoantibodies to RNA helicase A (RHA) were reported as a new serological marker of systemic lupus erythematosus (SLE) associated with early stage of the disease. Anti-RHA and other autoantibodies in Mexican SLE patients and their correlation with clinical and immunological features were examined.MethodsAutoantibodies in sera from 62 Mexican SLE patients were tested by immunoprecipitation of 35S-labeled K562 cell extract and enzyme-linked immunosorbent assay (anti-U1RNP/Sm, ribosomal P, β2GPI, and dsDNA). Anti-RHA was screened based on the immunoprecipitation of the 140-kDa protein, the identity of which was verified by Western blot using rabbit anti-RHA serum. Clinical and immunological characteristics of anti-RHA-positive patients were analyzed.ResultsAnti-RHA was detected in 23% (14/62) of patients, a prevalence higher than that of anti-Sm (13%, 8/62). Prevalence and levels of various autoantibodies were not clearly different between anti-RHA (+) vs. (-) cases, although there was a trend of higher levels of anti-RHA antibodies in patients without anti-U1RNP/Sm (P = 0.07). Both anti-RHA and -Sm were common in cases within one year of diagnosis; however, the prevalence and levels of anti-RHA in patients years after diagnosis did not reduce dramatically, unlike a previous report in American patients. This suggests that the high prevalence of anti-RHA in Mexican patients may be due to relatively stable production of anti-RHA.ConclusionsAnti-RHA was detected at high prevalence in Mexican SLE patients. Detection of anti-RHA in races in which anti-Sm is not common should be clinically useful. Racial difference in the clinical significance of anti-RHA should be clarified in future studies. |
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