|
|||||
|
|
A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454 |
|
| Authors: | Niall J Lennon Robert E Lintner Scott Anderson Pablo Alvarez Andrew Barry William Brockman Riza Daza Rachel L Erlich Georgia Giannoukos Lisa Green Andrew Hollinger Cindi A Hoover David B Jaffe Frank Juhn Danielle McCarthy Danielle Perrin Karen Ponchner Taryn L Powers Kamran Rizzolo Dana Robbins Elizabeth Ryan Carsten Russ Todd Sparrow John Stalker Scott Steelman Michael Weiand Andrew Zimmer Matthew R Henn Chad Nusbaum Robert Nicol |
| Affiliation: | 1. Genome Sequencing Platform, Broad Institute of MIT and Harvard, 320 Charles St, Cambridge, MA, 02141, USA 2. Network Control Engineering, Akamai Technologies Inc, 8 Cambridge Center, Cambridge, MA, 02142, USA 3. Engineering, Google Inc, 5 Cambridge Center, Cambridge, MA, 02142, USA 4. Genome Sequencing and Analysis Program, Broad Institute of MIT & Harvard, 7 Cambridge Center, Cambridge, MA, 02142, USA 5. Genomic Technologies, Joint Genome Institute, Walnut Creek, CA, 94598, USA |
| Abstract: | We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |