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Genetic transformation via particle bombardment of Catharanthus roseus plants through adventitious organogenesis of buds
Authors:Rafael Zárate  Johan Memelink  Robert van der Heijden  Robert Verpoorte
Affiliation:(1) Division of Pharmacognosy, Gorlaeus laboratories, Leiden/Amsterdam Center for Drug Research (LACDR), Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands;(2) Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands
Abstract:In vitro propagation of Catharanthus roseus was achieved using nodal explants. Bud induction was best on medium containing 1.0 mg benzyl aminopurine l–1. Hardening of rooted shoots to soil was very successful with 98% survival. Genetically transformed C. roseus plantlets were obtained after bombardment of nodal explants, which were then micropropagated, with DNA coated particles with green fluorescent protein (GFP) or beta-glucuronidase (GUS) reporter genes. Histological studies showed that the gene insertion method proved effective with many cells and different tissues displaying the reporter gene signals, showing that gene expressions were rather stable.
Keywords:  /content/n17x7016w1u60855/xxlarge946.gif"   alt="  beta"   align="  MIDDLE"   BORDER="  0"  >-glucuronidase  Catharanthus roseus  genetic transformation  green fluorescent protein  particle bombardment
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