Penetration of host cell lines by bacteria. Characteristics of the process of intracellular bacterial infection |
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Authors: | Jesus Gálvez Francisco Lajarin Pilar García-Peñarrubia |
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Institution: | (1) Laboratory of Physical Chemistry, Faculty of Science, 30071 Espinardo Murcia, Spain;(2) Department of Biochemistry and Molecular Biology B and Immunology, School of Medicine, 30071 Espinardo, Murcia, Spain |
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Abstract: | A model which describes the characteristics of the penetration of the cells by bacteria is presented. Since the process of
invasion is preceded necessarily by the step in which the bacteria adhere to the cells, the proposed model is based on the
expressions previously derived for the process of adhesion, which allow us to determine the number of attached bacteria under
different conditions. Thus, the model considers that invasion occurs irreversibly from attached bacteria to specific receptors
located on the cell surface with a rate coefficient=k
i
so that the invasive capacity in a given bacterium-host cell system is mainly determined by the value of this coefficient.
Once internalized, the bacteria can follow three different time courses, namely: 1) intracellular growth is hindered so that
the bacteria remain in stationary phase, 2) there is a lag phase during which the bacteria stay in stationary phase before
they are able to grow exponentially with a rate coefficient=k
c
, and 3) the bacteria exhibit a growth exponertial phase as they enter the cells. In turn, the time course followed by extracellular
bacteria also has a decisive influence on the process of invasion and, in this regard, unbound bacteria are considered either
in stationary or in exponential phase. Expressions for these different situations have been derived, and from them, procedures
to determine the levels of bacterial infection and for quantitative invasive data analysis are presented. |
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Keywords: | |
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