The induction of growth arrest in fibroblasts by SV40 T antigen |
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Authors: | Wang Xinwen Liu Yuan Dong Rui Jin Yan |
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Institution: | (1) Department of Oral Histology and Pathology, Center for Tissue Engineering, Stomatological College, The Fourth Military Medical University, Xi’an, 710032, China |
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Abstract: | DNA tumor viruses such as SV40, Ras and papillomaviruses are the most commonly used agents in immortalization of non-hematopoietic
cells, but the results are quite different. Some of them even lead instead to a senescence-like state. To verify the potential
of SV40 T antigen-mediated immortalization or properties and functions of it to regulate cell growth, human dermal fibroblasts
were cultured and then transfected with eukaryotic expressing plasmid psv3-neo which containing SV40 T DNA. We found that
expression of oncogenic SV40 T in human dermal fibroblasts resulted in growth, arrest, earlier than the occurrence of control
cell senescence, although telomerase was positive and cells grew faster than control ones in early stage following transfection.
These observations suggest that SV40 T antigen can activate growth arrest in human dermal fibroblasts under normal growth
condition instead of always prolonging the lifespan of fibroblasts. Moreover, high rate of cell division in early stage after
transfection may be associated with the expression of telomerase activity. |
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Keywords: | fibroblasts growth arrest SV40 telomerase |
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