Formation of interspecies fusants of <Emphasis Type="Italic">Agaricus bisporus</Emphasis> and <Emphasis Type="Italic">Agaricus bitorquis</Emphasis> mushroom by protoplast fusion |
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Authors: | Rana Inder Singh Kanojiya Aarti Sandhu Sardul Singh |
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Institution: | (1) Fungal Biotechnology and Invertebrate Pathology Laboratory, Department of Biological Sciences, R.D. University, Jabalpur, India |
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Abstract: | Interspecies fusants are formed between Agaricus bisporus and Agaricus bitorquis by protoplast fusion technique. Protoplasts were isolated and regenerated by using Novozyme 234 lytic enzyme. Twenty slow
growing isolates were separated from the protoplast regenerated colonies, which were assumed as homokaryons (putative homokaryons).
These twenty isolates were subjected to growth rate, colony morphology and spawn run studies for screening of true homokaryons.
Antifungal markers were developed for selection of fusants. |
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Keywords: | Agaricus bisporus Homokaryons Antifungal markers Protoplast fusion Fusants |
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