A bacterial high-affinity GABA binding protein: isolation and characterization |
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Authors: | Guthrie G D Nicholson-Guthrie C S Leary H L |
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Affiliation: | Department of Biochemistry, Indiana University School of Medicine, 700 Drexel Drive, Evansville, Indiana, 47712-9629, USA. gguthrie@usi.edu |
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Abstract: | A gamma-aminobutyric acid (GABA) binding protein (GBP) was isolated from a bacterial mutant which has high-affinity GABA binding characteristics comparable with the GABA(A) brain receptor in mammals. The GBP was partially purified and characterized and was shown to be a periplasmic protein of approximately 42,000 molecular weight. To determine the molecular weight, a bacterial GABA binding assay was used with SDS-PAGE. This procedure did not require large amounts or complete purification of protein and may be useful as a simple method in estimating the molecular weight of other bacterial binding proteins. |
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