Through-bond correlation of imino and aromatic resonances in 13C-,15N-labeled RNA via heteronuclear TOCSY |
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Authors: | Vladimír Sklená? Thorsten Dieckmann Samuel E Butcher Juli Feigon |
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Institution: | (1) Department of Physical and Theoretical Chemistry, Masaryk University, CZ-611 37 Brno, Czech Republic;(2) Institute of Scientific Instruments, Academy of Sciences of the Czech Republic, CZ-612 64 Brno, Czech Republic;(3) Department of Chemistry and Biochemistry, University of California, P.O. Box 951569, 90095-1569 Los Angeles, CA, USA;(4) Molecular Biology Institute, University of California, P.O. Box 951569, 90095-1569 Los Angeles, CA, USA |
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Abstract: | Summary Novel HCCNH TOCSY NMR experiments are presented that provide unambiguous assignment of the exchangeable imino proton resonances by intranucleotide through-bond connectivities to the (assigned) nonexchangeable purine H8 and pyrimidine H6 protons in uniformly 15N-, 13C-labeled RNA oligonucleotides. The HCCNH TOCSY experiments can be arranged as a two-dimensional experiment, correlating solely GH8/UH6 and GH1/UH3 proton resonances (HCCNH), of as three-dimensional experiments, in which additional chemical shift labeling either by GN1/UN3 (HCCNH) or by GC8/UC6 (HCCNH) chemical shifts is introduced. The utility of these experiments for the assignment of relatively large RNA oligonucleotides is demonstrated for two different RNA molecules.To whom correspondence should be addressed. |
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Keywords: | fMet-tRNA ATP Aptamer Multidimensional NMR |
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