Biology in a gray box: targeting the emergent properties of protein complexes: 2011 Yale Chemical Biology Symposium |
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Authors: | Wong Victor |
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Affiliation: | Department of Molecular, Cellular, and Developmental Biology, Yale School of Medicine, New Haven, Connecticut 06511, USA. victor.c.wong@yale.edu |
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Abstract: | At the 2011 Yale Chemical Biology Symposium, Jason Gestwicki presented a novel yet intuitive approach to drug screening. This method, which he termed "gray box" screening, targets protein complexes that have been reconstituted in vitro. Therefore, the gray box screen can achieve greater phenotypic complexity than biochemical assays but avoids the need for target identification that follows cell-based assays. Dr. Gestwicki's research group was able to use the gray box screen to identify myricetin as an inhibitor of the DnaK-DnaJ chaperone complex. This review will discuss Dr. Gestwicki's approach to identifying DnaK-DnaJ inhibitors as well as where the gray box screen fits among traditional techniques in drug discovery. |
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Keywords: | Jason Gestwicki biochemical screen cell-based screen gray box screen protein complexes emergent properties DnaK-DnaJ complex myricetin |
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