The metabolism of [Me-14C]choline in the brain of the rat in vivo |
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| Authors: | G B Ansell and Sheila Spanner |
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| Institution: | Department of Experimental Neuropharmacology, The Medical School, University of Birmingham |
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| Abstract: | Me-(14)C]Choline was injected intracerebrally into the adult rat, and its uptake into the lipids and their water-soluble precursors in brain was studied. The radioactivity could be detected only in the choline-containing lipids and was confined to the base choline. The results indicated that initial phosphorylation of the free choline followed by the formation of CDP-choline and the subsequent transfer of the phosphorylcholine to a diglyceride is one of the principal routes by which choline lipids in brain are formed. Further evidence for this was obtained in experiments in which either phosphorylMe-(14)C]choline or (32)P]orthophosphate was injected and the radioactivity in the choline-containing water-soluble and lipidbound components studied. |
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