|
|||||
|
|
Cloning and expression of two plant proteins: similar antimicrobial activity of native and recombinant form |
|
| Authors: | R Capparelli D Palumbo M Iannaccone I Ventimiglia E Di Salle F Capuano P Salvatore M G Amoroso |
| Institution: | (1) School of Biotechnological Sciences, University of Naples “Federico II”, Via Università 133, Portici, Naples, 80055, Italy;(2) Dipartimento di Biochimica e Biotecnologie mediche, University of Naples “Federico II”, Via Pansini 5, Naples, 80131, Italy;(3) Istituto Zooprofilattico Sperimentale per il Mezzogiorno, Via della Salute 2, Portici, Naples, 80055, Italy;(4) Department of Cellular and Molecular Pathology “L. Califano”, School of Biotechnological Sciences, University of Naples “Federico II”, Via Sergio Pansini 5, Naples, 80131, Italy |
| Abstract: | Antimicrobial peptides and proteins are being studied with increasing interest because of their broad range antimicrobial activity. Among plant antimicrobial proteins, the wheat seed polypeptides, puroindoline a and puroindoline b, are particularly interesting because of their established antibacterial activity. In this paper we describe different strategies used to clone His tagged and GST tagged puroindolines obtaining 1.5 mg recombinant protein from 1 l culture. The antimicrobial activity of recombinant and native puroindolines was comparable. |
| Keywords: | Antimicrobial activity Innate immunity Recombinant puroindolines Refolding Membrane damage |
| 本文献已被 PubMed SpringerLink 等数据库收录！ | |