Constitutive expression of a native human interferon-alpha 1 gene in Escherichia coli |
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| Authors: | I Ivanov N Markova D Bachvarov K Alexciev A Saraffova V Maximova I Tsaneva G Markov |
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| Affiliation: | Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia. |
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| Abstract: | 1. A plasmid for constitutive expression of the human interferon-alpha 1 (hIFN-alpha 1) gene in Escherichia coli is constructed on the basis of the cloning plasmid pBR322 using a strong synthetic promoter, synthetic ribosome binding site and a native hIFN-alpha 1 gene excised from a chromosomal clone. 2. The yield of recombinant hIFN-alpha 1 from E. coli LE392 cells transformed with the expression plasmid pJP1R9-hIFN-alpha 1 is evaluated to be 2-6 x 10(7) U/l bacterial culture for metabolic shaker and 6-8 x 10(7) U/l for fermentor. |
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