Human anti-rhesus D IgG1 antibody produced in transgenic plants |
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Authors: | Bouquin Thomas Thomsen Mads Nielsen Leif Kofoed Green Trine Hefsgaard Mundy John Dziegiel Morten Hanefeld |
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Institution: | (1) Department of Plant Physiology, Molecular Biology Institute, Copenhagen University, Øster Farimagsgade 2A, DK-1353 Copenhagen K, Denmark;(2) H:S Blodbanken, KI2033, Copenhagen University Hospital, Blegdamsvej 9, DK-2100 Copenhagen Ø, Denmark |
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Abstract: | Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a plantibody, we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD– mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs. |
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Keywords: | alloimmunization immunoglobulin Rhesus transgenic plants |
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