Development of microsatellite markers for the moss Ptychomitrium gardneri (Ptychomitriaceae) |
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Authors: | Liu Yan Ge Xue-Jun Sun Qi-Bao Cao Tong |
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Affiliation: | Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. |
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Abstract: | ? Premise of the study: Microsatellite markers were developed for Ptychomitrium gardneri to study population genetics of this eastern Asian-North American disjunct moss. ? Methods and Results: A total of 13 microsatellite markers were developed in Chinese populations of P. gardneri, using the Fast Isolation by AFLP of Sequences COntaining Repeats protocol. Eight of the markers showed polymorphism when assessed in a sample of four populations of 29 individuals from China. These markers amplified three to four alleles per locus. Five primers also amplified in P. linearifolium and P. wilsonii. ? Conclusions: These markers may be useful for further investigation of population genetics of P. gardneri. |
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