Quantification of phytochelatins in Chlamydomonas reinhardtii using ferrocene-based derivatization |
| |
| Authors: | Bräutigam Anja Bomke Susanne Pfeifer Thorben Karst Uwe Krauss Gerd-Joachim Wesenberg Dirk |
| |
| Institution: | Martin-Luther-Universit?t Halle-Wittenberg, Institut für Biochemie und Biotechnologie, ?kologische und Pflanzen-Biochemie, Kurt-Mothes-Stra?e 3, Halle (Saale), Germany. |
| |
| Abstract: | A method for the identification and quantification of canonic and isoforms of phytochelatins (PCs) from Chlamydomonas reinhardtii was developed. After disulfide reduction with tris(2-carboxyethyl)phosphine (TCEP) PCs were derivatized with ferrocenecarboxylic acid (2-maleimidoyl)ethylamide (FMEA) in order to avoid oxidation of the free thiol functions during analysis. Liquid chromatography (LC) coupled to electrospray mass spectrometry (ESI-MS) and inductively coupled plasma-mass spectrometry (ICP-MS) was used for rapid and quantitative analysis of the precolumn derivatized PCs. PC(2-4), CysGSH, CysPC(2-4), CysPC(2)desGly, CysPC(2)Glu and CysPC(2)Ala were determined in the algal samples depending on the exposure of the cells to cadmium ions. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
