Occurrence and Transmission of Facultative Endosymbionts in Aphids |
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Authors: | De-Qiao Chen Alexander H Purcell |
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Institution: | (1) Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720-3112, USA , US |
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Abstract: | The occurrence of a secondary bacterial symbiont (PASS) of
pea aphid, Acyrthosiphon pisum (Harris), was detected by polymerase
chain reaction (PCR) with specific nucleotide primers based on PASS 16S rDNA
nucleotide sequences from over 80% (50/57) of clones of pea aphid
collected from widely separated locations in California. PASS was also
detected by PCR in both red and green phenotypes of rose aphid,
Macrosiphum rosae (L.), but not in six other species of aphids
examined, including blue alfalfa aphid (A. kondoi Shinji). The
nucleotide sequences of the PCR-amplified, partial 16S rDNAs (1060 bp) from
pea aphid and rose aphid were identical and 99.9% similar to the
published 16S rDNA of PASS. PASS and a recently described new rickettsia of
pea aphid (PAR) were transmitted by needle injection of hemolymph from
positive pea aphid clones into negative clones and into blue alfalfa aphids.
Both PASS and PAR were maintained in the offspring of some of the injected
mother aphids via high rate of maternal transmission.
Received: 18 September 1996 / Accepted: 30 September 1996 |
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