A segregated model for plasmid content and product synthesis in unstable binary fission recombinant organisms |
| |
Authors: | Seo J H Bailey J E |
| |
Affiliation: | Department of Chemical Engineering, California Institute of Technology, Pasadena, California 91125. |
| |
Abstract: | Plasmid propagation in populations of unstable, binary fission recombinant organisms has been studied using a segregated, population balance mathematical model. Segregated models have the advantage of direct incorporation of basic information on mechanisms and kinetics of plasmid replication and segregation at the single-cell level. The distribution of cellular plasmid content and specific rates of plasmid gene expression have been obtained for several single-cell models of plasmid replication, partition, and gene expression. Plasmid replication kinetics during cell growth significantly influence the plasmid content distribution. In the case of transient growth of plasmid-containing and plasmid-free cells in partially selective medium, the degree of selection required for stable maintenance of plasmid-containing cells has been determined. Guidelines are presented for applicability of simpler, nonsegregated models and for evaluation of the parameters in these models based on single-cell mechanisms and associated parameters. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|