链霉菌FIM-0916产安福霉素的发酵条件优化 |
| |
作者姓名: | 杨煌建 张祝兰 黄小珍 郑卫 |
| |
作者单位: | 1.福建微生物研究所, 福州 350007;
2.江苏康缘制药有限公司, 江苏 连云港 222002 |
| |
基金项目: | 福建省自然基金计划项目(2012J01308)资助。 |
| |
摘 要: | 本文采用单因素和正交试验设计,对链霉菌 Streptomyces canus sp. FIM-0916产安福霉素的发酵培养基配方及发酵条件进行了优化。优化后的最佳培养基配方为:蔗糖1.5%,黄豆粉1.0%,组氨酸0.1%,KNO3 0.1%, CaCO3 0.1%。最佳发酵条件为:种子菌龄54 h,装液量120 mL/500 mL,接种量2%,发酵温度28℃,摇床转速250 r/min;最佳发酵时间为5 d。在该优化条件下,安福霉素的发酵效价比对照提高248%,为安福霉素的后续开发奠定了基础。
|
关 键 词: | 安福霉素 单因素考察 正交试验 发酵条件优化 |
收稿时间: | 2014-01-15 |
Optimization of Fermentation Conditions of Amphomycin-producing Strain Streptomyces canus sp. FIM-0916 |
| |
Authors: | YANG Huang-jian ZHANG Zhu-lan HUANG Xiao-zhen ZHENG Wei |
| |
Abstract: | The fermentation medium and conditions of Streptomyces canus sp. FIM0916 were optimizied for producing amphomycin by single-factor and orthogonal test design. The optimized medium formula is 1.5% sucrose, 3.5% soybean meal, 0.1% L-histidine, 0.1% KNO3 and 0.1% CaCO3;the optimal fermentation conditions are as follows: filling volume is 120 mL for a 500 mL shake flask, shakers rotating speed is 250 r/min, inoculum size is 2%, temperature 28℃, and cultivation time of seed 54 h; the optimal fermentation time are 5 d. Under optimized conditions, the titer of amphomycin was 248% higher than that of control, which laid the foundation for subsequent development on amphomycin. |
| |
Keywords: | amphomycin single factor experiment orthogonal experiment fermentation conditions optimization |
本文献已被 CNKI 等数据库收录! |
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |