灰霉病菌抗药位点及其分子检测方法研究进展

灰霉病由灰葡萄孢侵染所致，化学防治是目前最常用的治理方法，而随着杀菌剂的广泛使用，抗药菌株频繁出现。本文就近年来已研究报道的灰葡萄孢菌的抗药分子位点进行了系统总结，包括六大类杀菌剂，涉及5个基因；对灰霉病菌抗药位点的分子检测方法进行了综述，包括测序法、CAPS、ARMS、Tetra primer ARMS-PCR、AS real-time PCR、ASPPAA PCR和双杂交探针法，通过对不同检测方法进行比较分析，指出现有技术存在的问题并展望未来高通量的检测方法的发展方向。

Advances of Mutation Sites Associated with Fungicide Resistance in Botrytis cinerea and its Molecular Detection Methods

Grey mould is caused by Botrytis cinerea infection, which is usually controlled by chemical methods. Along with wide usage of fungicides, resistance to fungicides occured frequently among B. cinerea. This paper summarized mutation sites associated with fungicide resistance in B. cinerea, involving 6 kinds of fungicides and 5 genes. This paper also reviewed molecular methods to detect fungicide resistant sites, such as sequencing method，CAPS，ARMS，Tetra primer ARMS-PCR, AS real-time PCR, ASPPAA PCR and LightCycler technology, etc.. Through analyzing and comparing different kinds of methods，problems in using these detection techniques were pointed out and development of high throughtput detection method was prospected in the future.