| CHO细胞株开发技术策略探讨 |
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| 作者姓名： | 陶维红 秦民民 张哲如 |
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| 作者单位： | 上海津曼特生物科技有限公司, 上海 201210 |
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| 基金项目： | 上海市科学技术委员会“科技型中小企业技术创新资金”项目(1402H195100)资助。 |
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| 摘 要： | 主要介绍了单克隆抗体药物工业生产中宿主细胞选择、表达载体构建、转染方法、筛选技术、细胞培养工艺技术方法以及最后选定细胞株的标准等，结合单抗药物CHO细胞株开发和培养工艺的经验，对当前我国单抗CHO细胞株开发技术策略进行了探讨。
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| 关 键 词： | 单克隆抗体 CHO细胞 细胞株开发 培养工艺 |
| 收稿时间： | 2014-10-30 |
Strategy Discussion for CHO Cell Line Development |
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| Authors: | TAO Wei-hong QIN Min-min ZHANG Zhe-ru |
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| Institution: | Shanghai JMT-Bio Science & Technology Co. Lt., Shanghai 201210, China |
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| Abstract: | Several strategies for industrial production of monoclonal antibody drug were introduced in this paper, including host cell selection, construction of expression vector, transfection method, screening techniques, cell culture technology as well as the standard method of final cell lines selection. On the basis of the progress of monoclonal antibody drug CHO cell line development and cell culture process, current strategy for the development of CHO cell lines in monoclonal antibody technology were also discussed. |
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| Keywords: | monoclonal antibody CHO cell cell line development cell culture process |
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