Proteomic insights into adaptive responses of Saccharomyces cerevisiae to the repeated vacuum fermentation |
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Authors: | Jing-Sheng Cheng Xiao Zhou Ming-Zhu Ding and Ying-Jin Yuan |
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Institution: | (1) Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, P.O. Box 6888, Tianjin, 300072, People’s Republic of China |
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Abstract: | The responses and adaptation mechanisms of the industrial Saccharomyces cerevisiae to vacuum fermentation were explored using proteomic approach. After qualitative and quantitative analyses, a total of 106
spots corresponding to 68 different proteins were identified by matrix-assisted laser desorption/ionization time-of-flight
mass spectrometry. The differentially expressed proteins were involved in amino acid and carbohydrate metabolisms, various
signal pathways (Ras/MAPK, Ras–cyclic adenosine monophosphate, and HOG pathway), and heat shock and oxidative responses. Among
them, alternations in levels of 17 proteins associated with carbohydrate metabolisms, in particular, the upregulations of proteins involved in glycolysis, trehalose biosynthesis, and the pentose phosphate pathway,
suggested vacuum-induced redistribution of the metabolic fluxes. The upregulation of 17 heat stress and oxidative response
proteins indicated that multifactors contributed to oxidative stresses by affecting cell redox homeostasis. Taken together
with upregulation in 14-3-3 proteins levels, 22 proteins were detected in multispots, respectively, indicating that vacuum
might have promoted posttranslational modifications of some proteins in S. cerevisiae. Further investigation revealed that the elevations of the differentially expressed proteins were mainly derived from vacuum
stress rather than the absence of oxygen. These findings provide new molecular mechanisms for understanding of adaptation
and tolerance of yeast to vacuum fermentation.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Proteome Vacuum fermentation Adaptation Stress response Saccharomyces cerevisiae |
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