| Abstract: | Deoxycholate promotes phospholipase C degradation of endogenous phosphatidyl3H]inositol (Pl), phosphatidyl3H]inositol monophosphate (PIP) and phosphatidyl3H]inositol bisphosphate (PIP2) in rat cornea and human platelets. Hydrolysis of phosphatidyl3H]inositol significantly lags polyphospho3H]inositide degradation. Concomitantly, formation of 3H]inositol monophosphate (IP1) lags behind 3H]inositol bisphosphate (IP2) and 3H]inositol trisphosphate (IP3) production. These results demonstrate that rat cornea and human platelet phospholipase C cause a preferential hydrolysis of the endogenous polyphosphoinositides rather than phosphatidylinositol. |
