Adrenergic regulation of the uncoupling protein expression in foetal rat brown adipocytes in primary culture |
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| Authors: | A Porras M Fernández M Benito |
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| Affiliation: | Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Ciudad Universitaria, Madrid, Spain. |
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| Abstract: | The adrenergic and T3 modulation of UCP expression in non-proliferative foetal brown adipocyte primary cultures were studied. The UCP in the cultured cells was determined by immunological detection of the protein and by quantification of the mitochondrial GDP-binding. Our results showed a relative increase of 65-75% in UCP levels and 60-80% in the mitochondrial GDP-binding capacity under beta-adrenergic stimulatory conditions, while neither alpha 1-adrenergic agonists nor T3 showed an effect. |
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