Identification of several chloroplast DNA genes which code for the large subunit of Nicotiana Fraction I proteins |
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| Authors: | Katsuhiro Sakano S. D. Kung S. G. Wildman |
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| Affiliation: | (1) Department of Biology, Molecular Biology Institute, University of California, Los Angeles;(2) Laboratory of Physiology, Hyogo College of Medicine, Nishinomiya Hyogo, Japan |
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| Abstract: | Summary Electrofocusing of carboxymethylated, crystalline Fraction I proteins in polyacrylamide gels containing 8 M urea resolves the large subunit into three major peptides and the small subunit into one or more peptides. Electrofocusing of proteins isolated from leaves of the reciprocal, F1, hybrids: N. glutinosa x N. tabacum, N. glauca x N. tabacum, N. glauca x N. langsdorfii and the parental species confirms that coding information for the large subunit is inherited only by the maternal line whereas both parents contribute coding information for the small subunit. The analysis shows that one or more of the three peptides of the large subunit of Fraction I proteins from different Nicotiana species differ in isoelectric point and therefore serve as phenotypic markers for chloroplast DNA genes. |
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