Characterization and localization of atriopeptin in rat atrium |
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Authors: | J Tang H Fei CW Xie MZ Suen JS Han RJ Webber D Chang JK Chang |
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Institution: | Beijing Medical College, Beijing 100083, China Peninsula Laboratories, Inc., 611 Taylor Way, Belmont, CA 94002, USA |
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Abstract: | An antiserum specific for atriopeptin was used to characterize and localize atriopeptin-like immunoreactive material in rat atrium by radioimmunoassay and immunohistochemical techniques. The antiserum recognizes atriopeptin I, atriopeptin III, and -human atrial natriuretic polypeptide, but does not recognize met-enkephalin, cholecystokinin, dynorphin A (1–13), bradykinin, substance P, or β-endorphin. A high content of atriopeptin was found in crude extracts of rat atria, as compared to ventricles, and the atriopeptin-like immunoreactive material was found to be located exclusively in granules within atrial cardiocytes. Fractionation of the immunoreactive material by gel filtration and reverse-phase HPLC revealed the presence of multiple atriopeptins. |
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Keywords: | Atriopeptins Atrial natriuretic polypeptide Atrial natriuretic factor Radioimmunoassay Immunohistochemistry |
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