Evaluation of a simple Theileria annulata culture protocol from experimentally infected bovine whole blood |
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Authors: | Gharbi M Latrach R Sassi L Darghouth M A |
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Institution: | Laboratoire de Parasitologie, Université de la Manouba, Ecole Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Tunisie. gharbim2000@yahoo.fr |
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Abstract: | We have evaluated a new simple technique using whole blood from experimentally infected cattle for the isolation and cultivation of Theileria annulata. The study was carried out on 20 Holstein-Frisian bovines that had been experimentally infected with a virulent lethal dose of Theileria annulata. This technique has been compared to the classical peripheral blood monocyte isolation with Ficoll carried out on 22 experimentally infected Holstein-Friesian calves. The effectiveness of the reference technique was estimated to 86.4%, whilst the effectiveness of the new technique was 100%. Moreover, this new technique leads to time and money saving estimated to € 3.06 per sample. It decreases the contamination risks by reducing the steps of sample manipulation. |
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Keywords: | Theileria annulata cell culture blood Ficoll |
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