Chemical modification of alpha-chymotrypsin for obtaining high transesterification activity in low water organic media |
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Authors: | Kusum Solanki Shweta Shah |
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Institution: | Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India |
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Abstract: | Alpha-chymotrypsin was made more hydrophilic by modifying 11 (out of 16) ε-amino groups with pyromellitic dianhydride. The hydrophilic preparation was precipitated with n-propanol. This preparation gave significantly higher initial rates at the optimum aw (127.51 nmol mg?1 min?1 in n-octane and 21.30 nmol mg?1 min?1 in acetonitrile at aw=0.33) compared with the lyophilized preparation (53.50 nmol mg?1 min?1 in n-octane and 0.26 nmol mg?1 min?1 in acetonitrile at aw=0.97). FT-IR showed that the precipitate of modified alpha-chymotrypsin has a higher content of alpha-helices and beta-sheets compared to the lyophilized powder. |
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Keywords: | Alpha-chymotrypsin chemical modification low water organic media pyromellitic dianhydride transesterification reactions |
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