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Surface modification of polyacrylonitrile with nitrile hydratase and amidase from Agrobacterium tumefaciens |
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| Authors: | Gudrun Fischer-Colbrie Melanie Herrmann Sonja Heumann Arja Puolakka Amina Wirth Artur Cavaco-Paulo |
| Institution: | 1. Research Centre Applied Biocatalysis, Graz, Austria;2. Graz University of Technology, Environmental Biotechnology, Graz, Austria;3. Tampere University of Technology, Fibre Materials Science, Tampere, Finland;4. University of Applied Sciences in Northwestern Switzerland, Basel, Switzerland;5. University of Minho, Dept. of Textile Engineering, Guimaraes, Portugal |
| Abstract: | A new strain of Agrobacterium tumefaciens (BST05) was found to grow on polyacrylonitrile (PAN; 13C labelled) converting the polymer to polyacrylic acid as shown by solid state NMR. When cultivated in a medium containing acetonitrile the bacterium produced nitrile hydratase and amidase activity. Activity recovery after lyophilisation and enzyme stability was significantly enhanced in the presence of 5% sorbitol leading to half life times of 12, 72 and 154 days at 25°C, 4°C and –20°C. The enzymes were able to convert 1.1% of the nitrile groups of PAN-powder to the corresponding acids. PAN fabrics were mainly converted to the amides as shown by an 80% increase of the O/C ratio in ESCA analysis. These data were confirmed by cationic dyeing and FTIR-ATR analysis. |
| Keywords: | Agrobacterium tumefaciens nitrile hydratase polyacrylonitrile |